Document 0129
 DOCN  M9490129
 TI    [Studies of the resistance of the human immunodeficiency virus to
       azidothymidine. I. Kinetic studies of the interaction between reverse
       transcriptase and a series of its mutant forms with substrates and
       azidothymidine-5'-trisphosphate]
 DT    9411
 AU    Pokholok DK; Gudima SO; Esipov DS; Dobrynin VN; Memelova LV; Rechinskii
       VO; Kochetkov SN
 SO    Biokhimiia. 1994 May;59(5):739-47. Unique Identifier : AIDSLINE
       MED/94318764
 AB    Prolonged therapy of AIDS patients with azidothymidine results in the
       development of resistance to the drug. This phenomenon is accompanied by
       the appearance of point mutations in the pol gene coding for reverse
       transcriptase (RT). Kinetic studies of interactions of wild type RT and
       its forms containing the above-mentioned mutations with substrates and
       azidothymidine 5'-triphosphate have been carried out. Considerable
       differences in the affinities of RT and the mutants for RNA and DNA
       heteropolymer templates were established. The mutations did not affect
       the RT affinity for dNTP; however, its location influenced considerably
       the inhibition of the reaction with azidothymidine 5'-triphosphate.
 DE    Amino Acid Sequence  Base Sequence  Drug Resistance, Microbial/GENETICS
       DNA, Viral/METABOLISM  English Abstract  Genes, pol  Human  HIV-1/*DRUG
       EFFECTS/ENZYMOLOGY  Kinetics  Molecular Sequence Data  Point Mutation
       Reverse Transcriptase/GENETICS/*METABOLISM  RNA, Viral/METABOLISM
       Substrate Specificity  Thymine Nucleotides/*METABOLISM
       Zidovudine/*ANALOGS & DERIVATIVES/METABOLISM/*PHARMACOLOGY  JOURNAL
       ARTICLE

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